"I am devastated, not only to miss the World Open but the three largest tournaments of the year including the Qatar Classic and Hong Kong Open," said the world No8, who suffered a head injury following a fall two weeks ago in Milan, where she had been training.
"I hope to back on the World Tour again before Christmas," added the 30-year-old from Banbridge, near Belfast, in Northern Ireland.
Perry, who would have competed in her eighth successive World Open since 2000, was the centre of attraction at the 2006 World Open, staged in her home city Belfast last November.
The withdrawal has necessitated an amendment to the draw for the sport's biggest ever women's event, which will be staged adjacent to the Royal Palace (the Palacio Real) in the Spanish capital, from 23-27 October. The 32-draw qualifying championship will be staged at the city's Pallestra Squash Club from 21-22 October.
Former world champion Vanessa Atkinson takes Perry's place as the No8 seed, while fellow Dutch player Annelize Naude is promoted to 16th seed. Latasha Khan, the seven-time US champion who originally headed the qualification draw, now has a place in the main draw.
Revised 1st round draw:
 Nicol David (MAS) v Isabelle Stoehr (FRA)
 Shelley Kitchen (NZL) v Qualifier
 Natalie Grainger (USA) v Samantha Teran (MEX)
 Engy Kheirallah (EGY) v Qualifier
 Rachael Grinham (AUS) v Qualifier
 Alison Waters (ENG) v Qualifier
 Vicky Botwright (ENG) v Sharon Wee (MAS)
 Jenny Duncalf (ENG) v Latasha Khan (USA)
 Kasey Brown (AUS) v Qualifier
 Vanessa Atkinson (NED) v Alicia Alvarez Riaza (ESP)
 Laura Lengthorn-Massaro (ENG) v Qualifier
 Tania Bailey (ENG) v Jaclyn Hawkes (NZL)
 Annelize Naude (NED) v Raneem El Weleily (EGY)
 Omneya Abdel Kawy (EGY) v Qualifier
 Rebecca Chiu (HKG) v Qualifier
 Natalie Grinham (AUS) v Dominique Lloyd-Walter (ENG)